A cotton tipped swab is inserted into the cervical canal only 0.5 cm and
smeared on a slide and air dried for gram stain
placed in a transport medium for gonorrhoea culture
a second cotton tipped swab is inserted 0.5 to 1 cm into the cervical canal and kept in place for 10 to 15 seconds and rotated 1 to 2 times for chlamydia NAAT and gonorrhoea NAAT and mycoplasma genitalium NAAT testing
place in a transport container provided by the lab.
From the lateral vaginal wall with a cotton tipped swab:
smeared on a slide and air dried for gram stain for bacterial vaginosis and candida
placed in a transport medium for gonorrhoea culture and candida culture.
From the posterior fornix with a cotton tipped swab for:
pH performed on a pH strip at the time
wet preparation for trichomonas
smear on to a slide and place a drop of normal saline on slide - must be read immediately
trichomas vaginalis NAAT if indicated.
Self Collected Vaginal Specimens
Sample for chlamydia and gonorrhoea NAAT in asymptomatic female using cotton tipped swab and transported in a container provided by the lab.
These are taken if clinically indicated.
Proctoscopy should be performed to inspect for lumps, ulcers, pus, bleeding and specimen collection.
Swabs are taken for:
gonorrhoea culture (plate or transport medium)
chlamydia NAAT and gonorrhoea NAAT (dry or moistened cotton tipped swab)
herpes simplex NAAT if indicated.
These are taken routinely in sex workers and samples are collected.
Swabs are taken from each tonsillar fossa for:
gonorrhoea culture if suspected or NAAT positive
chlamydia and gonorrhoea NAAT for screening.
Dry or moist cotton tipped swabs taken from the ulcer base can be tested for herpes simplex virus (HSV) NAAT
swabs are transported in an empty transport container or in viral culture medium
syphilis NAAT using cotton tipped dry swab if indicated.
First Catch Urine
Can be performed if no endocervical swabs are available and samples:
should not pass urine for at least 2 hours before the sample collection
first 10 ml of urine is collected
voiding within 30 minutes prior to sample collection should be considered in interpreting results
tests for chlamydia NAAT, gonorrhoea NAAT, mycolplasma genitalium NAAT and trichomonas vagnilais NAAT as indicated.
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